GC Therapeutics is the first genome-wide cell landscape exploration company using an integrated synthetic biology and AI-driven platform for cell programming. Its patent-pending and proprietary pluripotent stem cell di?erentiation technology platform TFome™ was developed in Professor George Church's lab, a pioneer in synthetic biology, and allows for the development of unique cell therapies with significantly streamlined manufacturing, improved cell quality, e?ciency and speed. GC Therapeutics is based in Cambridge, MA. For additional information, please visit www.gc-tx.com.
Job Purpose
GC Therapeutics is seeking a talented and highly motivated Scientist for our Stem Cell team. The candidate will have strong expertise in human pluripotent stem cell biology. Reporting to the Head of Discovery Platforms, this individual will engineer novel cell therapies derived from human induced pluripotent stem cells (iPSCs) for regenerative and cell-based therapies. This role will involve bringing a new class of cellular therapies to patients using GCTx's Human TFome™ platform, a synthetic biology-driven technology to di?erentiate human stem cells. The scientist will be responsible for daily tasks involving the growth and di?erentiation of pluripotent stem cells and characterization of the target cell type.
Duties and Responsibilities
General Laboratory Roles:
Responsible for experimental design, data generation, analysis, and communication of results to supervisor, and team members.
Provide scientific and technical supervision to junior sta? or contract research organizations (CRO) for defined projects.
Maintain a detailed laboratory journal, summarize results and create presentations for internal group meetings.
Exemplify scientific curiosity and deep expertise with relevant literature and cutting-edge technologies to advance research directions.
Work with other members of the research team and within GCTx to accomplish company goals.
Follow all safe laboratory practices and company policies.
Experimental Techniques:
Cellular Biology:
Design and execute synthetic biology-based di?erentiation experiments of pluripotent stem cells.
Optimize, refine and scale di?erentiation protocols for candidate cell therapeutic products.
Analyze di?erentiated cell products and establish QC metrics.
Extensive experience with cell line engineering
Culture PSC lines including maintenance and analysis (e.g. cell culture, transfection, preparation of media, passaging, cryopreservation).
Perform cell type di?erentiation, characterization and experimental endpoint analysis including flow cytometry, ELISA and microscopy.
Molecular Biology:
Utilize techniques such as PCR, molecular cloning and plasmid isolation.
Characterize target cell types using next-generation sequencing methods (e.g. library preparation for bulk and single cell RNA-seq) and other gene expression analysis techniques.
Qualifications
Essential Qualifications:
Ph.D. degree in stem cell biology, cell biology, bioengineering, biochemistry, or related discipline and 5+ years of laboratory work experience in academia or industry.
Required experience with mammalian cell culture.
Proven experience in basic molecular biology laboratory techniques.
Strong work ethic with the ability to perform tasks independently and lead junior sta? e?ectively.
Proficient in analysis, presentation and documentation of scientific data as well as preparation of scientific reports.
E?ective communicator with excellent interpersonal skills.
Strong project management and organizational skills and ability to prioritize and multitask.
Ability to work in a laboratory environment approximately 75% of the time including some weekends.
Additional Preferred Qualifications:
Experience with pluripotent stem cells (e.g. hiPSCs).
Practical familiarity with stem cell di?erentiation protocols.
Experience in genome editing.
Proven track record and strong publications in stem cell or developmental biology.
Ability to analyze next generation sequencing or other genome-scale datasets.