Location: 10975 Torreyana Road, San Diego, CA, USA, 92121-1106
Duration: 12 Months
Description:
DUTIES AND RESPONSIBILITIES
Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry.
Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.
Responsible for transfection and expression of antibodies and recombinant proteins in cell lines, hybridoma tissue culture, and culture of various cell lines for testing. Specific requirement for hands-on experience with aseptic technique and cell-culture techniques.
Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met.
Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments. Will be expected to efficiently prioritize individual workload.
Involvement with scheduling, operational and maintenance duties required within the laboratory.
KNOWLEDGE AND SKILLS
Hands on experience designing, performing, and analyzing flow cytometry experiments, preferably on BD flow cytometers (LSRII, LSR Fortessa, Canto, Aria).
Proficient in tissue culture techniques; including transfection of cells for protein expression.
Software: BD FACS Diva, FlowJo, FCS Express, and Microsoft Office Suite.
Familiarity with molecular biology (DNA preparation)
Preferred: Familiarity with assay automation and automated liquid handlers.
Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.
Effective written and oral communication skills.
Demonstrated ability to follow established policies and procedures.
Demonstrated ability to keep neat, accurate, and complete records.
Effective analytical and problem-solving skills.
EDUCATION AND EXPERIENCE
A Bachelor's or Master of Science degree in cellular or molecular biology, immunology, cell biology or related scientific discipline and a minimum of 2-5 years of laboratory experience required. Flow cytometry and/or tissue culture experience required.