A Research Assistant position is available in the Oyoshi laboratory at Massachusetts General Hospital for Children to study the immune mechanisms of food allergy. The position will acquire a variety of skills that are required for in vitro and in vivo analysis of mouse models of allergic diseases, which will be instrumental for the development of the staff's career.
Candidates must have;
Excellent writing and communication skills in English
Previous experiences with handling and managing experimental animal colonies
Basic biology techniques, including sterile cell culture, gel electrophoresis, PCR, DNA/RNA extraction
Candidates who are and familiar with immunological techniques are desirable;
Primary cell isolation
ELISA
Quantitative PCR
Immunohistochemistry
Multi color flow cytometry
Animal models of human disease, preferably allergic inflammation
The priority will be given to applicants with an excellent background in Immunology. If applicable, publication(s) in international journals in Immunology area would be a plus. Please send a brief statement of your research experience and career goals (limit to one half page), curriculum vitae with bibliography, and names and contact information of three references in a single PDF file to Dr. Michiko Oyoshi at moyoshi@mgh.harvard.edu. Incomplete applications will not be reviewed.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Animal Colony
Manages experimental animal colonies: daily maintenance, breeding, crossing, and genotyping
Maintains weekly animal colony records and databases
Science
Plans, setups, and performs routine in vivo/in vitro experiments
Collects and organizes experimental samples and data
Maintains experimental records and databases
Prepares reagents and solutions
Assists lab members for conducting experiments
Troubleshoots assays, methods, equipment to obtain high quality data
Uses software programs to generate graphs and reports, including but not limited to, Word, Excel, Prism, PowerPoint, Adobe
Conducts literature searches
Management
Orders and maintains inventory of laboratory supplies/consumables
Manages institutional protocols including IACUC, IBC, and IRB
Ensures daily operations of laboratory, including maintaining equipment, protocols, research inventories, daily budget.
Performs administrative support duties as required
SKILLS/ABILITIES/COMPETENCIES REQUIRED:
Careful attention to details
Excellent organizational skills
Excellent communication skills
Ability to follow directions
Ability to work independently
Flexibility and willingness to complete tasks
Computer literacy
Knowledge of research protocols
Professional demeanor
Qualifications
EDUCATION:
Bachelor's degree required.
EXPERIENCE:
Animal handling: required
Basic biology techniques; required
WORKING CONDITIONS:
Works under general supervision in laboratory setting.
Supervisor regularly reviews progress of work.
EEO Statement
Massachusetts General Hospital is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.