some background in nucleic acids - mRNA technology
invitro transcription
basic understanding of chemistry
purification strategies for mRNA
ELISA, PCR
AKTA - preferred
MUST HAVE:
RNA biochemistry
understanding of nucleic acid
molecular biology
Day to Day:
planning experiments
invitro transcriptions
QCs
molecular biology work - cell testing of generated mRNAs
Key Responsibilities: • In vitro transcription to produce mRNA at different scales to support the platform development and therapeutic programs. • RNA Purification using affinity chromatography (AKTA) and other strategies for purification. • Quality control assays for mRNAs like electrophoresis, ELISA, qPCR, etc. • Perform cell-based assays, such as cell culture, transfection, and flow cytometry. • Work closely with the manager and cross-functional team members on multiple tasks with the ability to analyze, interpret, and present data. • Assist in ordering reagents from internal and external suppliers and communicating with CROs. • Generate reliable and reproducible data in a timely manner. Read and adapt scientific literature to accomplish assignments. Demonstrate broad and versatile technical expertise within discipline. • Maintain electronic laboratory notebooks (ELN) in compliance with the Company Laboratory Notebook Policy. • Stay updated and in compliance on all departmental and corporate training.
Key Qualifications: • MS in molecular biology, biochemistry, biomedical engineering or related field with 0-2 years of experience in biotherapeutics or genetic medicine or BS with a minimum of 3-5 years of relevant industry experience. • Hands-on experience with nucleic acid preparation, purification (AKTA), QC, and cell-based assays. • Working knowledge of related tools/software (SnapGene, Prism, FlowJo, Excel, PowerPoint etc.). • Self-motivated, hard-working, detail-oriented, resourceful, and able to work in a fast-paced team environment with demonstrated problem-solving skills. • High-quality written and oral communication skills and the ability to excel in a collaborative environment.