Isolation & characterization of human blood cells and in vitro cell assays
Contractor will request donor samples, isolate cells from human or monkey peripheral blood, set up cultures with isolated cells, perform cell stimulation or differentiation by test compounds, harvest cells and supernatants, prepare these samples for further analysis; run ELISA tests to study effector function of compounds; analyze and present the data generated by these experiments.
Qualifications
BS or MS & 3-5 years biochemistry lab experience
Primary cell isolation and culture experience
ELISA
In vitro cell assays
Flow cytometry
Basic lab techniques: sterile technique, pipetting samples, generating titrations/dilutions, dealing with cells, making buffers