Research Technician II at University of Pittsburgh in Pittsburgh, Pennsylvania

Job Description:

Research Technician II

A full-time Research Tech II position is available at the University of Pittsburgh, Ophthalmology Department in Dr. Liyun Zhang’s laboratory. The lab focuses on uncovering the mechanisms of retinal neuron degeneration and regeneration, with the ultimate goal of developing novel therapies for retinal degenerative disorders. Using CRISPR/Cas9 technology, the lab generates human retinal disease models in zebrafish to study key signaling pathways involved in disease progression and conduct in vivo phenotypic drug screening to identify potential therapeutic agents.

Requirements:

1 A Bachelor’s degree in Biology, Molecular Biology or related scientific field is required.

2 Previous research experience is preferred but not mandatory, especially with the following techniques and skills:

a. Zebrafish husbandry (e.g. general care, breeding, genotyping, phenotyping, drug treatments).

b. High resolution imaging (e.g. fluorescence and confocal microscopy).

c. Histological and immunological tissue processing.

d. Standard molecular biology techniques (e.g. PCR, cloning, RNA/DNA extraction)

3 Strong attention to details and excellent organizational skills.

4 Ability to work both independently and collaboratively within a diverse research team.

5 Enthusiasm for science and a proactive mindset to learn new techniques and contribute to innovative research.

Job Summary

Performs a variety of laboratory experiments and procedures. Prepares specimens. Oversees supply management. Operates and properly maintains equipment.

Essential Functions

The Research Technician will play a critical role in supporting the lab’s scientific endeavors by conducting experiments, maintaining laboratory organization, and ensuring the zebrafish facility operates smoothly. Key responsibilities include, but are not limited to:

1) Perform a variety of experiments, such as drug screening, histology, confocal imaging, zebrafish behavior assays, and generating transgenic models. Assist in data analysis and troubleshoot experimental challenges.

2) Maintain zebrafish stocks, monitor fish health, and organize zebrafish databases.

3) Maintain detailed, accurate and timely records of experimental data in organized logbooks. Document and analyze results to contribute to lab meetings and discussions.

4) Sterilize experimental materials, prepare solutions, maintain a clean and safe working environment.

5) Oversee supply management, including ordering, tracking and receiving materials and reagents.

Physical Effort

Must have good stereo vison to effectively use a stereo microscope for larval fish dissection and related tasks.

The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.

The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.

Assignment Category: Full-time regular

Job Classification: Staff.Laboratory Research Technician II

Job Family: Research

Job Sub Family: Laboratory

Campus: Pittsburgh

Minimum Education Level Required: Bachelor's Degree

Minimum Years of Experience Required: 1

Will this position accept substitution in lieu of education or experience: Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement.

Work Schedule: M- F 8:30am to 5:00pm

Work Arrangement: M- F 8:30am to 5:00pm

Hiring Range: TBD Based Upon Qualifications

Relocation_Offered: No

Visa Sponsorship Provided: No

Background Check: For position finalists, employment with the University will require successful completion of a background check

Child Protection Clearances: Not Applicable

Required Documents: Resume

Optional Documents: Cover Letter

PI256988931

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