Research Lab Technician II BWH Rheumatology at Partner's Healthcare in Boston, Massachusetts

Job Description:

Site: The Brigham and Women's Hospital, Inc.


At Mass General Brigham, we know it takes a surprising range of talented professionals to advance our mission-from doctors, nurses, business people and tech experts, to dedicated researchers and systems analysts. As a not-for-profit organization, Mass General Brigham is committed to supporting patient care, research, teaching, and service to the community. We place great value on being a diverse, equitable and inclusive organization as we aim to reflect the diversity of the patients we serve.


At Mass General Brigham, we believe a diverse set of backgrounds and lived experiences makes us stronger by challenging our assumptions with new perspectives that can drive revolutionary discoveries in medical innovations in research and patient care. Therefore, we invite and welcome applicants from traditionally underrepresented groups in healthcare - people of color, people with disabilities, LGBTQ community, and/or gender expansive, first and second-generation immigrants, veterans, and people from different socioeconomic backgrounds - to apply.



Job Summary
This position is in the laboratory of Ellen Gravallese, MD, Chief of the Division of Rheumatology, Inflammation, and Immunity. Our group is studying inflammation and osteoimmunology in the context of autoimmune diseases such as rheumatoid arthritis and spondylarthritis. In addition, we study innate immune mechanisms of autoimmune and autoinflammatory diseases. We utilize animal models of disease, human tissue samples, and in vitro experiments to study the pathways by which inflammation is mediated and also by which bone homeostasis is disrupted in disease states.


We are looking for a motivated and organized individual with hands-on experience working in the laboratory. The person filling this position will work closely with senior members of the lab, supporting them in various aspects of their ongoing research projects. In addition, it would be ideal if this candidate could also assist in our animal facility. The ideal candidate will have experience performing ELISAs, Westerns, primary cell culture, qPCR and histology. Experience in animal husbandry, genotyping (PCR), dissection and tissue harvesting, and organization of the animal database is also desirable, but not required. Finally, experience with analysis of bulk and single cell RNA sequencing data and the use of R would be an advantage.



Qualifications



Principal Duties and Responsibilities



Bench work


• Carries out the following:

• RT-PCR/qPCR


• ELISA


• Primary cell culture


• RNA extraction


• Westerns


• Microscopy


• FACs analysis


• Sample preparation for RNA sequencing


• Histological techniques: Immunohistochemistry, Immunofluorescence

• Responsible for the continual maintenance of the laboratory.


• Contributes to scientific literature.


• Attends regular meetings of the Principal Investigators to discuss improvements on the general operation of the laboratory and acts upon decisions made.


• Has working knowledge of databases/spreadsheets software (Microsoft Office)


• Responsible for maintenance of mouse colony, including weaning, ear tagging, genotyping by PCR, setting up breeding and record keeping.

Additional Duties/Skills/Competencies

• Keeping an up-to-date laboratory notebook


• Responsible for maintaining lab space, equipment, and supplies


• Ability to learn new laboratory techniques and to teach others as needed


• Ability to communicate well, both verbally and in writing


• Good organizational skills


• Ability to work closely with the research team


• Self-motivated and capable of trouble shooting and creative problem solving

Qualifications

• Bachelor's degree or equivalent required (suitable field preferred).

• Minimum of 1 year experience working in a laboratory.


• Relevant biology course work is preferred.


• Demonstrated organizational and interpersonal skills as required to work closely with a research team.


• Demonstrated competence with databases/spreadsheets and software packages.

Additional Job Details (if applicable)


Additional Job Description



Remote Type


Onsite



Work Location


60 Fenwood Road



Scheduled Weekly Hours


40



Employee Type


Regular



Work Shift


Day (United States of America)



EEO Statement:


The Brigham and Women's Hospital, Inc. is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.



Mass General Brigham Competency Framework


At Mass General Brigham, our competency framework defines what effective leadership "looks like" by specifying which behaviors are most critical for successful performance at each job level. The framework is comprised of ten competencies (half People-Focused, half Performance-Focused) and are defined by observable and measurable skills and behaviors that contribute to workplace effectiveness and career success. These competencies are used to evaluate performance, make hiring decisions, identify development needs, mobilize employees across our system, and establish a strong talent pipeline. Apply Now